Adult Stony Corals
This post is about Synchrotron based XRD, XRF, Raman data on Stylophora Pistillata
Adult stony coral samples:
Mature nubbins and new growth fronts
samples
Samples can be found partially:
CBP_Lab_Data(\tal-nas)(Z:)/Coral1/Maayan
Bold samples were used in the manuscript
SP - Stylophora Pistillata:
- SP3: ROI 1
- SP3: ROI 2
- SPS3a (lateral)
PP - Pocillopora Verrucosa:
- pp2: ROI 2
- pp3: ROI 2
- pp1: ROI 1
- pp1: ROI 2
- pp2: ROI 1
- pp2: ROI 3
- pp3: ROI 1
- pp5: ROI 1
- pp5: ROI 2
- pp5: ROI 3
- pp7: ROI 1
- pp7: ROI 2
Underwater pictures
Hagai Nativ: https://www. hagainativ.com
- Photography of corals Stillophora Pistillata \
- SEM image
X-ray Absorption Images
- Transmission images from Spec-files (BESSY, found wihtin one of the last columns)
- Transmission (T) transformed into Absorption (A):
A = 1-T
–> T was determined with Fiji, looking for the highest intensity value within histogram and this is defined then as “100% transmission = 1 in formula”:
a) 100% = 593751.44
As -A = T - 1 and T = our image:
with Fiji:
͛ 4; click on transmission image → process → math → substract 593751.44 → afterwards multiply with (-1)
XRD
In order to check if aragonite crystal intensity will change with directions all possible peaks of the spectrum will be plotted into 2D aragonite images. The following peaks are used:
| peak | channel | peak maximum | background | peak maximum |
|---|---|---|---|---|
| (111) | 315-385 (70) | 348 | 450-500 (50) | 475 |
| (021) | 385-445 (60) | 417 | 450-500 (50) | 475 |
| (012) | 800-840 (40) | 820 | 850-900 (50) | 875 |
| (200) | 999-1045 (46) | 1022 | 850-900 (50) | 875 |
| (112) | 1120-1160 (40) | 1140 | 1220-1270 (50) | 1245 |
| (022) | 1158-1213 (55) | 1182 | 1220-1270 (50) | 1245 |
| (211) | 1338-1382 (44) | 1360 | 1220-1270 (50) | 1245 |
| (122) | 1450-1495 (46) | 1474 | 1510-1560 (50) | 1535 |
| (221) | 1630-1695 (65) | 1667 | 1510-1560 (50) | 1535 |
| (041) | 1800-1860 (60) | 1830 | 1730-1780 (50) | 1755 |
| (132) | 1910-1980 (70) | 1948 | 2000-2030 (30) | 2015 |
RAMAN paper figure
## Compare with ORIGIN: peak width of peak with the highest intensity (number 1086). The peak width broadens the more the crystals (aragonite/calcite) lose the cristalinity and apear amorphous. I want to see if ACC is preesnt ##
→ plot RAMAN sprectrum
→ background reduction
→ within a range of 1000 - 2000 [1\cm] peak width was determined with the Voigt-function